alexa The Impact of HAART on Advanced Brain Aging: Implications for Mitochondrial Dysfunction and APP Processing | OMICS International
ISSN: 1948-5964
Journal of Antivirals & Antiretrovirals
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

The Impact of HAART on Advanced Brain Aging: Implications for Mitochondrial Dysfunction and APP Processing

Julia Campos de O’ Leary1 , Demian Obregon1,2,3, Frank Fernandez1,2, Jun Tan1,2,3 and Brian Giunta1,3*

1Neuroimmunology Laboratory, Department of Psychiatry and Behavioral Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL, USA

2Rashid Laboratory for Developmental Neurobiology, Department of Psychiatry and Neurosciences, Morsani College of Medicine, University of South Florida, Tampa, FL, USA

3James A. Haley Veterans’ Administration Hospital, Tampa, FL, USA

*Corresponding Author:
Brian Giunta M.D., Ph.D
Neuroimmunology Laboratory
Department of Psychiatry and Behavioral Neurosciences
Morsani College of Medicine
University of South Florida, Tampa, FL, USA
E-mail: [email protected]

Received Date: April 03, 2012; Accepted Date: May 14, 2012; Published Date: May 16, 2012

Citation: de O’ Leary JC, Obregon D, Fernandez F, Tan J, Giunta B (2012) The Impact of HAART on Advanced Brain Aging: Implications for Mitochondrial Dysfunction and APP Processing. J Antivir Antiretrovir S10. doi: 10.4172/jaa.S10-003

Copyright: © 2012 de O’ Leary JC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Antivirals & Antiretrovirals


Highly Active Antiretroviral Therapy (HAART) has significantly reduced AIDS-related morbidity and mortality. However the prevalence of HIV-1-Associated Neurocognitive Disorders (HAND) has been on the rise in the post- HAART era. A majority of the side effects of HAART can in part at least be attributed directly, or indirectly, to mitochondrial dysfunction. Indeed the rapid early clinical phase-in of HAART required dose de-escalations secondary to toxicities suggested to be related to drug side effects affecting mitochondria. Central to central nervous system (CNS) function is the amyloid precursor protein (APP), the parent protein from which amyloid-beta (Aβ) peptide is generated. Aβ generation and aggregation as plaques are well known in the age related dementia, Alzheimer’s disease (AD). It has been demonstrated that Aβ is common feature of the HIV infected brain as well. Further, reactive oxygen species (ROS) production is upregulated by HAART. Importantly, ROS promote β-secretase expression; a mechanism by which HAART may promote cognitive dysfunction, even in immune-competent HIV infected individuals.


Beta-secretase; APP; HAART; Amyloid-β; Microglia

Highly Active Antiretroviral Therapy, HIV Infection and Amyloid-Beta (Aß)

HIV-associated neuroinflammation is known to occur in even in the face of good virologic control with HAART [1]. As part of this neuroinflammation, the HIV itself promotes deposition of the same amyloid-β peptide (Aβ) found in Alzheimer’s disease (AD; for review see [2]). In HIV infected patients, Aβ immunoreactivity has largely been observed predominantly in the neuronal soma, dystrophic axons, and extracellular space [3-5]. Importantly, this Aβ deposition has been correlated with development of neurocognitive impairment [1]. In further support, Xu and colleagues [6] found, upon examination of autopsy brains of HIV Encephalitis (HIVE) and HIV seronegative cases, similar findings. Although intraneuronal Aβ immunoreactivity is also seen in aged control brains, it was significantly increased in HAART-treated HIVE brains. Extracellular Aβ deposition was also found in HAART-treated brains from patients with HIV-associated dementia (HAD) but HAART-untreated HAD brains show only intraneuronal Aβ accumulation [6]; indicating some mechanistic role of HAART in Aβ deposition. The prevalence of this intraneuronal Aβ staining was about 30-40%, and extracellular Aβ was present in 4-13% of HIV-infected brains, with a significantly higher percentage of extracelluar Aβ present in HAART-treated patients [5]. Importantly, Brew and colleagues found cerebrospinal fluid (CSF) Aβ 1-42 and tau levels correlate with HIV-associated cognitive impairment (HAND) [1].

It is possible that extracellular Aβ (eAβ) and intracellular amyloid-beta (iAβ) are present and interact in a cyclic pathway [7,8]. Neuronal loss is a late event in neurodegeneration. Many changes, including synapse dysfunction, electrophysiological properties and morphological atrophy, occur prior to neuronal loss [9]. Although iAb and its accumulation may be an early event prior to senile plaque and neurofibrillary tangles (NFT), iAb may alter cellular functions that would subsequently lead to neuronal loss [7].

iAβ is widely detected in neuronal cells and mainly produced by neurons, but glial cells also produce it in the normal human brain [10]. The iAβ accumulation precedes eAβ deposits and plaque formation. In animal models, iAβ accumulation precedes morphological deficits [11,12]. Aβ is generated by the sequential enzymatic cleavage of amyloid precursor protein (APP), and processing may occur within the endoplasmic reticulum (ER) intermediate compartment [13].

There are several hypothetical pathways that may result in iAb accumulation [7]. First, iAβ may be formed in the ER, recognized as a misfolded protein, and then translocated to the cytosol where it is ubiquitinated and sent to the proteasomes for degradation [14]. Since this degradation process decreases with aging, or medication toxicities, inefficient clearance of Aβ could result in iAβ accumulation. Secondly, secreted Aβ may be internalized into endosomes [15,16], increasing the membrane permeability of lysosomes [17], and thus, promote leaks into the cytosol. Thirdly, iAβ may occur due to passive leakage along any component of the secretory pathway. Fourth, eAβ passively diffuses through the plasma membrane into the cytosol or is actively brought in by surface receptors [18]. Finally, oxidative DNA damage induces iAβ accumulation resulting p53 mRNA increase in the nuclei leading to Bax and caspase-6 activation and subsequent execution of the cell apoptotic pathway [19].

Importantly, cellular toxicity of iAβ may be cell-type specific, because it induces cell death only in human primary neurons, but not in human primary astrocytes, murine neuroblastoma cells (NT2a), LaN1 or M17 cells [19]. It also appears that the Aβ oligomers, but not fibrils, may be the more toxic species [19], and that the iAβ toxicity may be attributed to these Aβ oligomeric forms.

Thus it is not surprising that accumulation of iAβ is correlated with apoptotic cell death. Alterations in axonal structure and transport may account for the iAβ neurotoxicity and its role in memory function. Accumulation of iAβ increases the number of Golgi apparatus elements, lysosomes and lipofuscin bodies in the hippocampus [20], and also leads to axonopathy with the formation of axonal spheroids as well as myelin ovoids.

There are at least two forms of eAb, high molecular weight insoluble Aβ fibrils that accumulate in the extracellular space as senile plaques [21] and soluble forms of Aβ that correlate with synaptic dysfunction and cognitive decline [22,23] which include: (a) soluble small globular structures of synthetic Aβ termed Aβ-derived diffusible ligands (ADDLs) [24,25], (b) curvilinear structures of protofibrils [26], and (c) Aβ oligomers; especially nanomers and dodecamers [27].

The positron emission tomography (PET) tracer 11C-labeled Pittsburgh Compound-B (11C-PIB) specifically binds fibrillar Aβ plaques and can be detected [29]. In a recent case-control study, cognitively unimpaired, HIV infected patients had an 11C-PiB scan within 2 years of concomitant CSF studies and neuropsychometric testing. As would be expected, none of the HIV+ participants had fibrillar amyloid plaques as assessed by increased 11C-PiB Mean Cortical Binding Potential (MCBP) or binding potential within four cortical regions [30]; lending further support to the findings of Brew and colleagues [1]. In the following review we suggest it is possible Aβ biogenesis is increased by the upregulation of β-secretase (BACE) through mitochondrial reactive oxygen species (ROS) activity imparted by HAART.

Disruption of Mitochondrial Function by HAART

Highly active antiretroviral therapy (HAART) has significantly reduced AIDS-related morbidity and mortality. However the prevalence of HIV-1-associated neurocognitive disorders (HAND) has been on the rise in the post-HAART era [31-33]. HAART, and particularly the nucleoside reverse transcriptase inhibitors (NRTI) (especially didanosine, stavudine, zalcitabine, and to a lesser extent zidovudine (AZT), abacavir and lamivudine [3TC]), has been positively correlated with serious adverse reactions.

Most of these can in part at least be attributed directly or indirectly to mitochondrial dysfunction [34-37]. Mitochondria are key organelles in energy production in all nucleated human cells. This energy, in the form of ATP, is produced through the oxidative phosphorylation pathway. Furthermore, mitochondria perform an array of other biological functions and modulate factors involved in cell apoptosis [38].

NRTIs have traditionally been suggested to be major culprit in HAART-induce mitochondrial toxicity due to their ability to inhibit Pol-γ, the DNA polymerase responsible for the synthesis of mitochondrial DNA [34,39,40]. Nevertheless, accumulating evidence points to a more complex relationship between these organelles and NRTIs, as well as non-nuceloside reverse transcriptase inhibitors (NNRTIs) such as efavirenz (EFV) and Protease Inhibitors (PI). The rapid early clinical phase-in of HAART required dose de-escalations secondary to toxicities suggested to be related to drug side effects on mitochondria [38]. For example, it has been shown the HAART drug combination of zidovudine (AZT) and the PI, indinavir (IDV) can disrupt the function and viability of endothelial cells due to loss of mitochondrial membrane potential; partially reversible with the thiol antioxidant N-acetylcysteine amide [32]. In adipocytes from HAART treated patients, it has also been shown that NRTI administration correlated positively with mitochondrial DNA depletion [41,42] suggesting an etiology for the lipodystrophy imparted by HAART. There are also coherent experimental and clinical arguments for the existence of mitochondrial toxicity following perinatal exposure to AZT, alone or in combination with the NRTI 3TC [43,44]. Further it has been demonstrated that placental tissue of HIV-1-infected HAARTexposed pregnancies undergoes mitochondrial DNA depletion with secondary respiratory chain compromise [45] and also that HAART treated pregnant mothers can have children with mitochondrial dysfunction [46]. It has also been found in synaptosomes and isolated mitochondria, as well as human subjects [47,48] that the NRTI, didanosine, can induce oxidative stress, cause the release of cytochrome c, reduce the levels of anti-apoptotic proteins, and increase the levels of pro-apoptotic proteins [49].

Elevation of ROS, APP Processing and Aß Biogenesis

Central to CNS neural function is the amyloid precursor protein (APP), the parent protein from which amyloid-beta (Aβ) peptide is generated. Aβ generation and aggregation as plaques are the hallmark pathology of Alzheimer’s Disease (AD; [15,50-53]). The peptides have been evidenced to be neurotoxic, as they are reported mediators of inflammation [54,55], and oxidative stress [56]. Aβ peptides are produced via the amyloidogenic pathway of APP proteolysis, which involves the actions of β and γ-secretases [15]. Initially, β-secretase (BACE) cleaves APP, creating an Aβ-containing carboxyl-terminal fragment known as β-C-terminal fragment (β-CTF) [57,58]. In the human brain Aβ40 is the predominant form whereas Aβ42 represents about 10% Aβ in the brain and has a greater propensity to form neurotoxic oligomeric and aggregated species (for review, see [59]). NFT, like amyloid, have also been implicated as a central pathological feature of AD. They are misfolded and hyperphosphorylated tau, a microtubule formation protein element (for review see [60]). The accumulation of Aβ can adversely affect disrcrete molecular pathways, thus facilitating tau phosphorylation, aggregation, and accumulation of abnormal hyperphosphorylated tau. Aβ and abnormal hyperphosphorylated tau synergize to accelerate neurodegenerative mechanisms involved in aging, metabolism, cellular detoxification, and mitochondrial dysfunction, resulting in neuritic plaque formation [61]. Levels of BACE - 1 are increased in vulnerable regions of the AD brain, but the underlying mechanisms are not known.

Importantly, it has been demonstrated that ROS stimulate β-secretase expression [62], suggesting a mechanism by which HAARTinduced ROS promotes β-secretase transcription, thereby promoting production of pathological levels of Aβ linked cognitive dysfunction in AD which could be applied to HAND. Indeed deposition of Aβ is common feature of HIV infection [5,63,64]. Mitochondrial dysfunction has been observed in postmortem brains of AD patients [65] just as in HAART-treated HIV-infected patients [66]. Indeed, mitochondrial dysfunction in both AD [67-69] and HAART-treated patients [66,70-74] is characterized by elevated ROS generation [75], decreased electron transport chain activity, most markedly in cytochrome c oxidase, and altered Krebs cycle enzyme activities [32,45,76,77]. It has been suggested that mitochondria play a pivotal role in the irreversible loss of neuronal function and in the neuronal cell death that occurs during the pathogenesis of both conditions [49,78].

Aging, Chronic HAART Administration and Development of Cognitive Deficits

Despite this dramatic improvement in AIDS related morbidity and mortality, high rates of HAND continue to be reported [6,85-88]. Indeed HAND, chronic HIV infection, and aging may all possibly contribute to the development of new forms of neurodegenerative processes based on mitochondrial dysfunction, ensuing upregulation of BACE1, which in turns promotes amyloidogenic APP processing and formation Aβ plaques. All of this would be reflected in accelerated aging-like neurocognitive deficits. The life span increase imparted by HAART also brings patients to an age in which AD is more common and the development of adverse effects of long term medication with HAART may present [89,90].

In support, we recently found that antiretroviral compounds might increase Aβ generation and decrease its clearance by inhibiting microglial phagocytosis, affecting both, amyloidogenic fronts, generation and clearance [90]. Specifically, we found high levels of Aβ1-42 peptide remaining in the cultured media after N9 microglial cells were treated with antiretrovirals alone or in combination upon completion of phagocytosis assay [90]. In addition, a majority of the compounds tested also significantly reduced levels of phagosomal (cell associated) Aβ1-42 suggesting that HAART can cause microglial phagocytosis inhibition [90]. The most significant amyloidogenic effects were observed with combined HAART, suggesting certain HAART medications may have additive amyloidogenic effects when combined [90]. Recent clinical studies [87,91] further suggests that in well controlled HIV infection, HAART can have a negative effective on cognitive function. It was found, from 167 HIV patients with a median nadir CD4 count of 436 cells/mm3 and 4.5 median years on HAART, that neurocognitive functioning actually improved after HAART discontinuation [91]. This improvement continued over the course of the 96-week followup of the study among the patients remaining off HAART [91]. They observed continued improvement from 48 weeks out (third testing) from the study, indicating that the improvements were not attributed to practice or learning effects. Antiretrovirals that enter the CNS were widely represented in their HAART regimens. They also noted a lack of substantial neurocognitive improvement with resumption of HAART [91]. This study is interesting in that removal of the HAART from patients under good viremic control improved cognition. One would expect that resumption of HAART may again induce cognitive problems however this was not the case. Therefore follow-up studies will need to be performed to determine the underlying mechanism of this phenomenon. Most recently it was shown that efavirenz (EFV) is associated with cognitive disorders in even asymptomatic HIVinfected patients [87]. Further, a randomized controlled study [92] found subjects receiving EFV-containing regimens for 48 weeks showed less improvement from baseline on instruments examining speed of information processing and executive function than patients not on EFV, suggesting EFV use may promote neurocognitive decline. This is also supported by findings of Robertson et al. 2010 [91], in which patients with preserved immune function on EFV regimens showed greater improvement on Trails-Making Tests A and B and WAIS digit symbol after antiretroviral treatment interruption than the non-EFV control group. Of note, the trail-making test measures visual attention and task switching. The instrument consists of two parts in which the subject is instructed to connect a set of 25 dots as rapidly as possible while still maintaining accuracy. It is able to provide data regarding visual search speed, scanning, speed of processing, mental flexibility, and executive functioning [93]. Additionally it is sensitive to detecting several cognitive impairments [93] and both tests in this study have been found to be sensitive and specific to detecting HAND [94,95]. The lack of observed further cognitive decline upon HAART reinitiation in these patients may be related to not following the cohort long enough for the chronic effects of HAART in the CNS to re-initiate. It might also be due to limited power. As it has been suggested that earlier initiation of HAART may improve clinical outcomes, the effect of HAART vs. that of unchecked HIV replication on cognitive function will require further prospective studies [91,96].

Finally, considerable neuroinflammation coupled with mononuclear phagocyte activation has been found in HAART medicated brains, particularly in the hippocampus. Anthony and colleagues [97] found a high level of microglial/macrophage activation that is comparable with the levels seen, pre-HAART, in HIVE and AIDS cases. This result was maximal in the hippocampus where microglial/macrophage upregulation in the HAART-treated group exceeded that seen in HIVE. In the basal ganglia, HAART-treated cases showed significantly higher levels of CD68-positive microglia/ macrophages than in control brains, and in the hippocampus levels were significantly higher than those seen in control cases, pre-HAART AIDS, and presymptomatic brains. Overall there is a significant degree of ongoing neuroinflammation in HAART-treated patients, particularly in the hippocampus. This may pose a threat for the future health of individuals maintained long-term on HAART therapy [97]. Neuroinflamaton is also a feature of both aging and AD (for review see [98]). We and others have shown this resultant elevated secretion of pro-inflammatory cytokines including IFN-γ, TNF-α, and IL-1β can increase Aβ generation and reduce Aβ clearance [6,98,99,100].

In summary it is clear that at least certain HAART regimens, especially those containing EFV, have the potential to cause cognitive decline, despite good control of the HIV itself [87]. Further, it is known that CNS Aβ production is a common feature of the HAART treated brain [3,5] which correlates with cognitive deficits [1]. Therefore, as the aging and efficaciously treated HIV-infected population continues to grow, there will likely be a need to phase in less toxic HAART regimens [101] and/or develop adjunctive neuroprotective, or prophylactic treatments for these undesirable side-effects.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Recommended Conferences

Article Usage

  • Total views: 11720
  • [From(publication date):
    specialissue-2013 - Mar 19, 2018]
  • Breakdown by view type
  • HTML page views : 7953
  • PDF downloads : 3767

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version