The Importance of Cytogenetic in the Study of Triatomines - An Editorial Review
Kaio Cesar Chaboli Alevi* and Maria Tercília Vilela de Azeredo Oliveira
Department of Biology, Institute of Biosciences, Humanities and the Exact Sciences, Sao Paulo State University, Brazil
- *Corresponding Author:
- Kaio Cesar Chaboli Alevi
Instituto de Biociências, Letras e Ciências Exatas
IBILCE – UNESP,Rua Cristovão Colombo, 2265
Jardim Nazareth 15054-000 – São José do Rio Preto, SP- Brazil
Tel : (17) 32212380 Ramal: 2378
E-mail : [email protected]
Received date: November 10, 2013; Accepted date: November 12, 2013; Published date: November 20, 2013
Citation: Alevi KCC, de Azeredo Oliveira MTV (2013) The Importance of Cytogenetic in the Study of Triatomines - An Editorial Review. Entomol Ornithol Herpetol 2:e105. doi: 10.4172/2161-0983.1000e105
Copyright: © 2013 Alevi KCC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Visit for more related articles at Entomology, Ornithology & Herpetology: Current Research
Triatomines belong to the Hemiptera order and Triatominae subfamily . The Triatominae subfamily is be composed of 146 species hematofagous [2,3] and potential vectors of Trypanosoma cruzi, etiologic agent of Chagas disease.
Classic cytogenetic studies means of technique Lacto-acetic orcein and C-banding, are considered important tools taxonomic in Triatominae subfamily, as they allow for the clarification of many problems, as the reorganization of Brasiliensis subcomplex [4-6], in the description and revalidation of species [7,8] in the study of cryptic speciation  and differentiation of species morphologically related [10,11]. However, in 2005, molecular cytogenetic studies, using the technique of in situ hybridization (FISH) have been initiated in triatomines .
The probes used in FISH (45S and 28S) allow taxonomic and evolutionary studies in these vectors. Currently, of the 146 described species only 46 present studies with the FISH technique, distributed in eight genus: Psammolestes (1), Dipetalogaster (1), Eratyrus (1), Panstrongylus (3), Mepraia (2), Meccus (3), Triatoma (23), Rhodnius (12) [12-17]. Intraspecific variation was also observed with FISH in T. infestans and R. ecuadoriensis [16,17]. New studies are necessary to the understanding of evolution of these insects.
Even after the emergence of molecular cytogenetics of triatomine, classical cytogenetics is still of great taxonomic importance because they are simple techniques that allow solving big problems, as mentioned above. Thus, increasingly the cytogenetics of triatomines is an important cytotaxonomic and evolutionary tool directly aiding in the understanding of these insects of great importance in public health.
- Lent H, Wygodzinsky P (1979) Revision of the Triatominae (Hemiptera:Reduviidae) and their significance as vector of Chagas’s disease. Bull Am Mus Nat Hist. 163: 123-520.
- Alevi KCC, da Rosa JA, de Azeredo Oliveira MTV (2013) Mini Review: Karyotypic Survey in Triatominae Subfamily (Hemiptera, Heteroptera). Entomol Ornithol Herpetol. 2: 106.
- Jurberg J, Cunha V, Cailleaux S, Raigorodschi , Lima MS, et al. (2013). Triatoma pintodiasi sp. nov. do subcomplexo T. rubrobaria (Hemiptera, Reduviidae, Triatominae). Rev Pan-Amaz Saude. 4: 43-56.
- Alevi KC, Mendonça PP, Pereira NP, Rosa JA, Oliveira MT (2012) Karyotype of Triatoma melanocephala Neiva and Pinto (1923). Does this species fit in the Brasiliensis subcomplex? Infect Genet Evol 12: 1652-1653.
- Alevi KC, Mendonça PP, Succi M, Pereira NP, Rosa JA, et al. (2012) Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector. Genet Mol Res 11: 4278-4284.
- Alevi KC, Mendonça PP, Pereira NP, Guerra AL, Facina CH, et al. (2013) Distribution of constitutive heterochromatin in two species of triatomines: Triatoma lenti Sherlock and Serafim (1967) and Triatoma sherlocki Papa, Jurberg, Carcavallo, Cerqueira & Barata (2002). Infect Genet Evol 13: 301-303.
- Frías-Lasserre D (2010) A new species and karyotype variation in the bordering distribution of Mepraia spinolai (Porter) and Mepraia gajardoi Frías et al (Hemiptera: Reduviidae: Triatominae) in Chile and its parapatric model of speciation. Neotrop Entomol 39: 572-583.
- Jurberg J, Galvão C, Lent H, Monteiro F, Macedo C, et al. (1998) Revalidação de Triatoma garciabesi Carcavallo, Cichero, Martínez, Prosen & Ronderos (1967) (Hemiptera-Reduviidae). Ent Vect. 5: 107-122.
- Silistino-Souza R, Alevi KCC, Castro NFC, Freitas MN, Papa MD, et al. (2013). Entoepidemiology of Chagas disease in northwest São Paulo and cytogenetic analysis of its main vector, Triatoma sordida (Hemiptera: Triatominae). Gen Mol Res., in press.
- Alevi KC, Mendonça PP, Pereira NP, Fernandes AL, da Rosa JA, et al. (2013) Analysis of spermiogenesis like a tool in the study of the triatomines of the Brasiliensis subcomplex. C R Biol 336: 46-50.
- Alevi KCC, Mendonça PP, Pereira NP, Rosa JA, Azeredo-Oliveira MTV (2013) Heteropyknotic filament in spermatids of Triatoma melanocephala and T. vitticeps (Hemiptera, Triatominae). Taylor & Francis Online
- Severi-Aguiar GD, de Azeredo-Oliveira MT (2005) Localization of rDNA sites in holocentric chromosomes of three species of triatomines (Heteroptera, Triatominae). Genet Mol Res 4: 704-709.
- Severi-Aguiar GD, Lourenço LB, Bicudo HE, Azeredo-Oliveira MT (2006) Meiosis aspects and nucleolar activity in Triatoma vitticeps (Triatominae, Heteroptera). Genetica 126: 141-151.
- Morielle-Souza A, Azeredo-Oliveira MTV (2007) Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization. Genet Mol Res. 6: 713-720.
- Bardella VB, Gaeta ML, Vanzela AL, Azeredo-Oliveira MTV (2010) Chromosomal location of heterochromatinand 45S rDNA sites in four South American triatomines (Heteroptera:Reduviidae). Comp Cytogenet. 4: 141-149.
- Panzera Y, Pita S, Ferreiro MJ, Ferrandis I, Lages C, et al. (2012) High dynamics of rDNA cluster location in kissing bug holocentric chromosomes (Triatominae, Heteroptera). Cytogenet Genome Res 138: 56-67.
- Pita S, Panzera F, Ferrandis I, Galvão C, Gómez-Palacio A, et al. (2013) Chromosomal divergence and evolutionary inferences in Rhodniini based on the chromosomal location of ribosomal genes. Mem Inst Oswaldo Cruz 108.