alexa UPLC-MS/MS Quantification of Fenspiride in Human Plasma | OMICS International
ISSN: 0975-0851
Journal of Bioequivalence & Bioavailability
Like us on:
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

UPLC-MS/MS Quantification of Fenspiride in Human Plasma

Yuriy V Pidpruzhnykov1,2*, Valerii E Sabko1, Volodymyr V Iurchenko1 and Igor A Zupanets2

1Bioanalytical laboratory “Clinfarm”, 3 Shevchenko Street, Irpin, Kiev Oblast, 08200, Ukraine

2National University of Pharmacy, 27 Pushkinskaya Street, Kharkiv, 61057, Ukraine

*Corresponding Author:
Yuriy V Pidpruzhnykov
Bioanalytical laboratory “Clinfarm”
3 Shevchenko Street, Irpin
Kiev Oblast, 08200, Ukraine
Tel: +380-442-575-201
Fax: +380-443-907-372
E-mail: [email protected]

Received Date: April 24 2013; Accepted Date: June 20, 2013; Published Date: June 25, 2013

Citation:Pidpruzhnykov YV, Sabko VE, Iurchenko VV, Zupanets IA (2013) UPLCMS/MS Quantification of Fenspiride in Human Plasma. J Bioequiv Availab 5: 185-190. doi: 10.4172/jbb.1000156

Copyright: © 2013 Pidpruzhnykov YV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at Journal of Bioequivalence & Bioavailability


A new simple and rapid ultra-performance liquid chromatography - tandem mass spectrometry (UPLC-MS/ MS) method has been developed and validated for the quantification of fenspiride in human plasma. Following a simple protein precipitation with acetonitrile, the analyte and internal standard (bupivacaine) were separated on an ACQUITY UPLC ® BEH С18 column (50 mm×2.1 mm, 1.7 μm) using a gradient mixture of acetonitrile and water (both containing 0.2% formic acid) as a mobile phase at flow rate 0.4 mL/min. The retention time of fenspiride and bupivacaine was 0.64 and 0.87 min, respectively. Detection was operated in positive mode by monitoring the transitions of m/z 261→105 for fenspiride and m/z 289→140 for bupivacaine. The lower limit of quantification was 2 ng/mL by using 200 μL aliquots of plasma. The range of quantification of fenspiride was 2-500 ng/mL. Within-run and between-run precision of analysis was <9.5%, accuracy ranged from 91.5% to 112.4%, average recovery from 99.3% to 101.9%. The analyte was stable in human plasma following three freeze/thaw cycles and for 5 months following storage at -70°C. The method was successfully applied to the bioequivalence study of generic and brand coated tablets of fenspiride


Fenspiride; Human plasma; Respiratory tracts


Fenspiride {8-(2-phenylethyl)-1-oxa-3,8-diazaspiro[4.5]decan-2- one.HCl} is an effective drug in case of acute and chronic inflammatory diseases of otolaryngological system and respiratory tracts. It is also used as a complex therapy for bronchial asthma, allergic rhinitis and other symptoms of allergy within otolaryngological system and respiratory tracts, respiratory manifestations of measles, flu and symptomatic therapy of whooping cough.

The aim of this work covered development and validation of the method for quantification of fenspiride in human plasma during bioequivalence study of coated tablets Inspiron® produced by JSC “Kievmedpreparat”, Ukraine and the brand one Eurespal® produced by “Les Laboratories Servier”.

The first method for the quantification of fenspiride in plasma and urine which was described in 1989 [1] included liquid-liquid extraction of analyte from the biomatrix with a mixture of organic solvents, reextraction with aqueous acid and back-extraction with an organic solvent. The extract was evaporated to dryness and reconstituted in the mobile phase. The quantification of fenspiride was run by HPLC method on the reversed-phase column, while electrochemical detection was used for concentration range from 2 to 100 ng/mL and ultraviolet detection was used for concentration range from 100 to 1000 ng/mL. With the help of this quite complex method the phase I pharmacokinetic study of fenspiride in 12 healthy volunteers was conducted in 1993 [2]. In the paper [3] fenspiride and its metabolites in equine plasma and urine were detected with the help of capillary gas chromatography-mass spectrometry method. The method is also characterized by complex sample preparation which covers solid-phase extraction, analytes elution from the sorbent by strong base in ethyl acetate, evaporation to dryness under N2 and obtaining of trimethylsilyl derivates of fenspiride.

The selective LC-MS/MS method was developed for more than 250 basic drugs screening including fenspiride in the supernatant of enzyme hydrolysed equine urine after extraction on the short Oasis HLB® column [4]. One more method based on gas chromatographymass spectrometry was developed for narcotics and stimulants in equine urine screening [5]. Fenspiride and other analytes were extracted from biomatrix by organic solvents, extracts were evaporated to dryness under N2, after that the derivates of N-methyl-Ntrimethylsilyltrifluoroacetamide were obtained.

In the article [6] fenspiride was determined in human plasma using the liquid-liquid extraction of fenspiride and the internal standard in 1-octanol, followed by direct injection of large volume aliquot (75 μL) of 1-octanol containing the analytes in the reversed-phase chromatography column and MS/MS detection.

This article describes development and validation of a new rapid UPLC-MS/MS method for the quantification of fenspiride in human plasma. The lower limit of quantification (LLOQ), was 2 ng/mL by using 200 μL aliquot of human plasma and simple precipitation procedure. The developed method satisfied all regulatory requirements [7,8] and was successfully applied to the bioequivalence study of coated tablets of fenspiride.

Materials and Methods

Reagents, solvents and materials

Fenspiride (Fn) reference standard (99.5% purity) produced by Erregierre S.p.A., batch #0108001 , Inspiron® coated tablets 80 mg, batch #41209 produced by JSC “Kievmedpreparat”, Ukraine and Eurespal® coated tablets 80 mg, batch #833149 produced by “Les Laboratories Servier”, were provided by JSC “Kievmedpreparat”, Ukraine.

The internal standard (IS) bupivacaine hydrochloride (Bv) (99.9% purity) was obtained from Sigma-Aldrich. Chemical structures of Fn and Bv are shown in Figure 1.


Figure 1: Chemical structures of fenspiride and bupivacaine.

Methanol and acetonitrile (99.9%, for HPLC) were obtained from Sigma-Aldrich, formic acid (98-100%) was obtained from Riedel-de- Haen.

Water was purified by “Elix-35” (Millipore, USA), final purification - by “Milli-Q Gradient” (Millipore, USA). All other chemicals were of analytical grade. Drug-free human plasma from healthy volunteers was kindly provided by JSC “Biopharma”, Ukraine, each batch was accompanied by the quality certificate and stored at -30°C prior to use.

Chromatography was performed in gradient mode. Solvent A: acetonitrile/water/formic acid (10:90:0.2, v/v/v), solvent B: acetonitrile/ formic acid (100:0.2, v/v).

Equipment and methods

The LC-MS/MS analyses were performed using the Waters Acquity UPLC® system with the Waters ACQUITY UPLC® BEH C18 columns (50 mm×2.1 mm, 1.7 μm) (both Milford, MA, USA) in combination with the Quattro Micro API triple quadrupole mass spectrometer (Micromass, Manchester, UK) equipped with an electrospray interface. MassLynx 4.1 software was used for instruments control and data processing.

Chromatography was performed at 30°C at a flow rate of 0.4 mL/ min with a run time of 1.2 min. The starting mobile phase consisted of 94% solvent A and 6% solvent B and the linear gradient run over 0.7 min to 40% A, 60% B. Then composition was set immediately at 100% B and held constant for 0.1 min, followed back to the start conditions. The autosampler was kept at 10°C.

Detection of the ions was performed in the Multiple Reaction Monitoring Mode (MRM). The detection parameters are listed in the Table 1. Collision gas pressure (argon) was set at 2.7 μbar. The MRM parameters were determined by means of the autotune option.

Ion source
Ion Mode Electrospray+
Capillary voltage 200 V
Desolvation temperature 500°C
Source temperature 130°C
Desolvation gas (nitrogen) flow 800 l/h
MRM parameters Fn Bv
Precursor ion (m/z) 261.13 289.17
Product ion (m/z) 104.93 140.12
Collision energy (eV) 24 18
Cone voltage (V) 30 25

Table 1: Ion source and MRM parameters.

Standard solutions and quality control (QC) samples

The stock solutions of Fn and Bv (400 and 200 μg/mL, respectively) were prepared in methanol. Appropriate dilutions with acetonitrile/ water/formic acid (20:80:0.2, v/v/v) were made to produce Fn working solutions of 20; 60; 200; 500; 1000; 2000; 3000; 5000 ng/mL. These solutions were used to prepare the calibration standards. Fn working solutions of 60; 1000; 3000 ng/mL were used too for preparation of low, medium and high QC samples, respectively.

The stock solution of Bv was diluted with acetonitrile/formic acid (99:1, v/v) to produce working solutions of IS (50 ng/mL).

The stock solutions were stored at -35°C, the working solutions - approximately at 5°C for a month. The calibration standards and QC samples were prepared by spiking 200 μL of blank human plasma with the appropriate amount of the analyte (20 μL).

Sample preparation

200 μL of plasma sample and 20 μL acetonitrile/water/formic acid mixture (20:80:0.2, v/v/v) were added to 1.5 mL polypropylene centrifuge tube and vortexed. Then, 500 μL of IS working solution were added (containing 25 ng of Bv, i.e. 125 ng/mL final IS plasma concentration) and the mixture was vortexed again. The samples were centrifuged for 10 min at 20000 rpm on a benchtop centrifuge (Sigma 3K30C). The supernatant (200 μL) was transferred to the glass autosampler vials containing 1000 μL of 0.1% formic acid. The vials were capped, vortexed, and 4 μL were injected into UPLC-MS/MS system.

Method validation

Full validation of the developed method was done in accordance with the requirements [7,8]. The method was validated for selectivity, LLOQ, calibration curve, within-run and between-run precision and accuracy, recovery, matrix effect and stability.

Six batches of blank plasma were used to determine the selectivity. Fn was added to plasma in quantity which corresponded to LLOQ (2 ng/ mL); IS stock solution was added to achieve 125 ng/mL concentration in plasma. The given solutions as well as the blank plasma solutions which were prepared in triplicate as described above were analyzed, and the peaks areas from the corresponding chromatograms were compared.

The LLOQ was assessed using 5 solutions which were prepared on the blank plasma with the nominal concentration of 2 ng/mL Fn in plasma. We analyzed the given solutions and the blank plasma samples which were prepared according to the section “Sample preparation”.

The calibration graph was evaluated by using 8 calibration standards with Fn concentrations in plasma of 2, 6, 20, 50, 100, 200, 300, and 500 ng/mL.

The accuracy and the precision of the method were evaluated using the model biosamples which were prepared in the group of five for each of four Fn concentrations in blank plasma equivalent: 2 ng/mL (LLOQ), 6 ng/mL (low QC), 100 ng/mL (medium QC), and 300 ng/ mL (high QC). The experiment was conducted on three different days.

Within-run and between-run precisions were calculated using the formula %R.S.D. = (S.D./M)×100, where M is the mean of the experimentally determined concentrations and S.D. is the standard deviation of M. Accuracy was defined as the percent relative error (% RE) and was calculated using the formula % RE = (E-T)×100/T, where E is the experimentally determined concentration and T is the theoretical concentration.

The extraction recovery of Fn and Bv from plasma was determined at 3 concentrations: low, medium and high QC samples (3 replicates) by comparing the areas of extracted samples with the blank plasma extracts fortified with the drug post extraction [7].

The matrix effect was investigated using 6 batches of human plasma. For the analyte and the IS, the matrix factor (MF) was calculated for each batch of plasma by calculating the ratio of the peak area in the matrix presence (measured by analyzing the blank matrix spiked with the analyte at a concentration of 6 ng/mL after extraction (3 LLOQ) to the peak area in the mobile phase) [8].

Stability of the analyte in the studied matrix was evaluated using triplicates samples of the low and high QC samples which were analyzed immediately after preparation and after the applied storage conditions [7,8]. The short-term stability was examined following sample defrosting and 4-hour incubation at room temperature. The freeze-thaw stability was examined after three freeze-thaw cycles. The long-term stability was determined by analyzing five aliquots at nominal concentrations of 6 and 300 ng/mL stored at -70°C for 150 days. The stability of the samples processed in autosampler was examined after 22 hours. The stock solutions stability was assessed after 1 month storage at a temperature of -35°C, working solution after the same storage period at a temperature of 2 to 7°C.

Application for bioequivalence study

The bioequivalence study for coated tablets of fenspiride 80 mg was conducted in accordance with the Declaration of Helsinki, GCP and GLP requirements. The protocol and associated informed consent forms were reviewed and approved by The Central Ethical Committee of Ministry of Healthcare of Ukraine, and the informed consent forms were signed by the volunteers prior to the study initiation. Twenty four healthy volunteers (aged 18-52) were enrolled in this study. The volunteers met the requirements of inclusion/exclusion criteria. They had no history of cardiovascular, hepatic, renal, gastrointestinal, hematologic and nervous or any acute or chronic diseases or drug allergy, and had stopped using any drugs 2 weeks prior to the study enrollment. The volunteers did not receive any concomitant medication within 2 weeks prior to screening as well as during the study. Physical examination and laboratory tests showed no abnormal findings. The volunteers were randomized in two groups of 12. The cross over design was applied to this study when one subgroup was administered the study drug followed by administration of the reference drug, another subgroup was administered vice-versa. All subjects were administered a single dose of 80 mg after overnight fasting. The subjects were required to refrain from smoking, alcohol, caffeine and were under direct medical supervision at the study site.

14 blood samples were drawn from each subject at each study time point. The blood sampling was conducted according to the following schedule: 0 h (pre-dose), 30 min, 1 h, 2 h, 4 h, 5 h, 6 h, 7 h, 8 h, 12 h, 24 h, 36 h, 48 h, and 72 h post-dose. The samples were transferred in the lithium heparinized tubes (9 mL volume) and centrifuged within 1 min after collection. The centrifugation was conducted in the temperaturestabilized rotary chamber at 10°C for 5 min at 4000 g. The plasma was divided into two aliquots (the first one was for the analysis, the second one was for back-up) but the volume was not less than 1.3 mL for each aliquot, which were transferred into the pre-labeled cryotubes. The plasma was kept at -70°C at the study site and at the bioanalytical laboratory as well. The time period between blood collection and plasma transfer into -70°C freezer did not exceed 12 min.

23 volunteers completed the study, 1 subject withdrawn the consent prior to Period 1 because of adverse event (headache) treatment. All required results were included into pharmacokinetic parameters assessment. Totally, 655 frozen samples were submitted to the bioanalytical laboratory for assessment.

The data analysis of pharmacokinetic parameters was performed by using the Win-Nonlin professional software version 5.2 (Pharsight, Mountain View, CA, USA) using non-compartmental approach to the assessment model of “NCA Model 200”. AUC0-72 values (t being the time of the last plasma concentration measured) were calculated by the linear trapezoidal rule. The AUC from 0 to infinity (AUC0-∞) was calculated by the software. The first order rate constant, Kel, was estimated by the linear regression of time versus log of the concentration. The terminal half-life (T1/2) was also calculated.

The bioequivalence was confirmed by the approach based on the statistically assessed 90% confidence intervals for the ratio of the average Cmax and AUC0-t parameters for the drug A and B. The drugs were considered as bioequivalent if 90% confidence interval was within 0.80-1.25 (80-125%) for the ratio of the average Cmax and AUC0-t parameters.

Results and Discussion

Liquid-liquid extraction without following evaporation and reconstitution of samples was used in the previously mentioned article [6]. Fenspiride and internal standard (trimetazidine) were extracted from plasma in 1-octanol and directly injected of large volume aliquot on the column. Total time required for chromatography of one sample in this method was 7 min (stop time of the method) due to a relatively long time period needed for washing off the immiscible diluent from the column. This period is much more than stop time of the method which we developed (1.2 min). The total time needed for the analysis is extremely important for the pharmacokinetic studies when large numbers of biosamples need to be analyzed.

Samples preparation and chromatographic conditions

Acetonitrile containing IS and 1% of formic acid has proved quite effective as an agent for plasma proteins precipitation at agent/plasma ratio 2.5:1 (v/v). The obtained supernatants were diluted with acidified water for harmonization with the starting chromatographic conditions.

The gradient profile was optimized to achieve good peak shapes and a short run time for the compounds, as well as to avoid the ion suppression.

The typical chromatograms of a blank, a spiked plasma sample with Fn at LLOQ and IS, and a plasma sample from a healthy volunteer 12 h after oral administration and spiked IS are shown in Figure 2.


Figure 2: Typical MRM chromatograms of fenspiride (left panel) and bupivacaine (IS, right panel) in human plasma: (A) blank plasma sample; (B) blank plasma sample spiked with fenspiride (2 ng/ml, LLOQ) and IS (125 ng/ml); (C) plasma sample from a volunteer at 12 h after administration of Inspirone® tablets (427 ng/ml of fenspiride found).

The data registration time was 1.2 min. Such a short period of time allowed chromatography of each study sample to be conducted twice. The analysis of one sample took less than 3 minutes, taking into account the sampling in load ahead mode. The Fn concentration was calculated for the mean value of two parallel injections response. Significant time reduction on chromatography by the UPLC-MS/MS method is an indisputable advantage of the new approach as compared with the traditional HPLC-MS/MS. It is essential for conducting the bioequivalence study.

Matrix effect, selectivity and recovery

The matrix effect study showed that the ion suppression of Fn and Bv is practically absent under the selected conditions (Table 2). The IS normalized MF (MFN) also was calculated by dividing the MF of the analyte by the MF of the IS [8]. The results obtained satisfy the requirements [8], since RSD of MFN calculated from 6 batches of matrix (3.0%) is much less than 15%.

Batch of blank plasma MFFn MFBv MFN
  1.01 1.03 0.976
2 1.01 1.09 0.923
3 1.03 1.07 0.964
4 1.00 1.09 0.921
5 1.06 1.07 0.985
6 1.06 1.08 0.980
Mean 1.03 1.07 0.958
RSD, % 2.6 2.1 3.0

Table 2: Matrix factor of Fn (MFFn), Bv (MFBv) and the IS normalized matrix factor of Fn (MFN) in human plasma (n=6).

During the selectivity estimation the mean “noise” peak area for each of six batches of plasma was less than 20% of the LLOQ for the analyte: 4.6, 5.3, 4.4, 4.2, 3.2 and 2.3% respectively.

The extraction recovery of Fn was consistently high within a wide range of concentrations (6-300 ng/mL): 99.3- 101.9%. The mean extraction recovery of Bv was equally high: 101.6%.

Calibration curve and lower limit of quantification

The calibration curve was generated from 8 calibration standards prepared from blank plasma over the Fn concentration range of 2 ng/ mL to 500 ng/mL. The results of the blank sample and zero sample were not taken into consideration when constructing the calibration function.

Partially, linear regression equation with the weighting factor 1/ concentration did not satisfy the acceptance criteria [7] because of unacceptable deviation of LLOQ standard. At the same time, quadratic model with the weighting factor 1/concentration continuously described the calibration curve adequately.

The mean coefficients of the regression equation Y = aX2 + bX + c had the following values (n=7):

a = -3.480.10-7, b = 0.002638; c = 0.0001417.

The mean value of the coefficient of determination (R2) was 0.999. The back calculated concentrations of the calibration standards complied with the acceptance criteria [7,8].

The validated value of LLOQ was 2 ng/mL with the mean signal/ noise ratio 46.0. Five independent standards of the mentioned concentration and five blank biosamples were analyzed in order to obtain this value. The back calculated concentrations of the LLOQ standards were found with precision and accuracy within ± 15% (Table 3).

    Spiked TMHP concentration (ng/ml) Measured TMHP concentration (ng/ml) RSD (%) RE (%)
Within-run Day 1, n=5 1.990 1.894 3.8 -4.8
5.970 5.945 6.1 -0.4
99.50 91.46 3.7 -8.1
298.5 276.0 6.0 -7.6
Day 2, n=5 1.990 2.238 4.4 12.4
5.970 6.168 3.8 3.3
99.50 91.36 1.9 -8.2
298.5 273.2 1.6 -8.5
Day 3, n=5 1.990 2.040 9.5 2.5
5.970 6.089 6.2 2.0
99.50 95.78 3.5 -3.7
298.5 295.1 2.5 -1.1
Between-run 3 days, n=15 1.990 2.057 9.3 3.4
5.970 6.067 5.3 1.6
99.50 92.87 3.7 -6.7
298.5 281.4 5.0 -5.7

Table 3: Within-run and between-run precision and accuracy.

Precision and accuracy

Quintuple determination which was conducted in the five model biosamples for each of four chosen concentrations has shown that accuracy and precision were better (Table 3) than it was prescribed by the acceptance criteria [8].


The obtained stability results (RE < 12.9% for all kinds of stability and concentration ranges) indicate that the data comply with the acceptance criteria [7,8]. It was established that the model biosamples were stable for 150 days of storage at the temperature not higher than -70°C. Storage time of the real biosamples did not exceed 43 days.

Bioanalytical study

Mean plasma concentration - time profiles for 23 volunteers after oral administration of the test and the reference drug are presented in Figure 3.


Figure 3: Mean plasma concentration-time profile of fenspiride following administration of 80 mg tablets of Eurespal® (reference) and Inspirone® (test) to 23 healthy volunteers.

Inspiron®, 80 mg coated tablets, produced by JSC “Kievmedpreparat”, Ukraine, has the pharmacokinetic profile similar to Eurespal, 80 mg coated tablets, produced by “Les Laboratories Servier”. The statistical analysis on all acquired data was performed employing the ANOVA algorithm (Win-Nonlin professional software version 5.2). The main pharmacokinetic parameters and its standard deviations are listed in Table 4.

Parameters Test drug Reference drug
Cmax(ng/ml) 332.5 ± 70.6 284.5 ± 49.4
Tmax (h) 7.61 ± 2.57 7.74 ± 2.80
AUC0-24 (h∙ng/ml) 9084 ± 2020 8123 ± 1817
AUC0-∞ (h∙ng/ml) 9487 ± 2185 8472 ± 1954
AUC0—24/ AUC0-∞ (%) 95.97 ± 2.00 96.09 ± 2.03
Kel (h-1) 0.0485 ± 0.0068 0.0494 ± 0.0068
T1/2 (h) 14.58 ± 2.22 14.31 ± 2.21

Table 4: Mean pharmacokinetic parameters of Fn for 23 volunteers after administration of the study and reference coated tablets (mean ± S.D., n = 23).

The data of the bioequivalence assessment of the given drugs are listed in Table 5. These findings suggest that the drugs are bioequivalent.

Drug Сmax, ng/ml max А/Сmax В, % Δ Сmax, % AUC0-t, h∙ng/ml *AUC0-t А/AUC0-t В, % Δ AUC0-t,%
Inspiron (A) 332.5 115.9 109.3-123.0 9084 112.3 106.4-118.5
Eurespal (B) 284.5 8123

Table 5: Results of bioequivalence study of tablets Inspiron® relative to tablets Eurespal®.


The new method of quantification of fenspiride in human plasma using UPLC-MS/MS was developed. The method proved to be rapid and simple. The performed method validation has indicated the conformity of its properties with the established requirements. The method has been successfully applied to the bioequivalence study of generic and brand 80 mg coated tablets of fenspiride.


We thank to JSC “Kievmedpreparat”, Ukraine for funding the study.


Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Recommended Conferences

Article Usage

  • Total views: 11909
  • [From(publication date):
    July-2013 - Mar 20, 2018]
  • Breakdown by view type
  • HTML page views : 8095
  • PDF downloads : 3814

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version