| A: Intra day precision and accuracy of doxycycline |
| S. No. |
Run ID |
Back calculated concentration (ng/mL) |
| LLOQ QC |
LQC |
MQC |
HQC |
| Nominal |
51.000 |
140.000 |
2250.000 |
3750.000 |
| 1 |
1_PA01_DI01 |
52.054 |
135.890 |
2138.233 |
3894.285 |
| 54.541 |
147.006 |
2143.167 |
3655.231 |
| 52.213 |
132.561 |
2277.448 |
3602.057 |
| 50.892 |
144.378 |
2163.433 |
3753.031 |
| 55.299 |
135.245 |
2234.456 |
3671.566 |
| 51.913 |
145.184 |
2201.595 |
3703.162 |
| Mean |
52.819 |
140.044 |
2193.055 |
3713.222 |
| SD |
1.709 |
6.164 |
55.283 |
101.907 |
| %CV |
3.24 |
4.40 |
2.52 |
2.74 |
| %Accuracy |
103.57 |
100.03 |
97.47 |
99.02 |
| N |
6 |
6 |
6 |
6 |
|
| B: Inter day precision and accuracy of doxycycline |
| Sr. No. |
Run ID |
Back calculated concentration (ng/mL) |
| LLOQ QC |
LQC |
MQC |
HQC |
| Nominal |
51.000 |
140.000 |
2250.000 |
3750.000 |
| 1 |
2_PA01_DI01 |
52.054 |
135.890 |
2138.233 |
3894.285 |
| 54.541 |
147.006 |
2143.167 |
3655.231 |
| 52.213 |
132.561 |
2277.448 |
3602.057 |
| 50.892 |
144.378 |
2163.433 |
3753.031 |
| 55.299 |
135.245 |
2234.456 |
3671.566 |
| 51.913 |
145.184 |
2201.595 |
3703.162 |
| 2 |
3_PA02_ABSOLUTE RECOVERY |
55.405 |
137.067 |
2247.117 |
3606.683 |
| 54.149 |
143.092 |
2316.983 |
3601.448 |
| 53.430 |
140.503 |
2172.055 |
3740.801 |
| 50.001 |
142.142 |
2188.159 |
3601.126 |
| 51.115 |
137.198 |
2062.007 |
3651.585 |
| 51.173 |
137.447 |
2155.949 |
3515.588 |
| 3 |
4_PA03 |
45.613 |
131.408 |
2076.568 |
3638.903 |
| 51.750 |
131.422 |
2125.969 |
4044.558 |
| 47.921 |
131.279 |
2320.304 |
3616.170 |
| 47.615 |
137.615 |
2097.308 |
3607.019 |
| 49.777 |
134.943 |
2113.591 |
3958.929 |
| 48.176 |
127.819 |
2141.915 |
3785.726 |
| Mean |
51.280 |
137.344 |
2176.459 |
3702.659 |
| SD |
2.758 |
5.442 |
76.688 |
139.659 |
| %CV |
5.38 |
3.96 |
3.52 |
3.77 |
| % Accuracy |
100.55 |
98.10 |
96.73 |
98.74 |
| N |
18 |
18 |
18 |
18 |
|
| Table 2: Intra day and inter day precision and accuracy of doxycycline. |
