Parameter VD-2 VD-3 25(OH)VD-2 25(OH)VD-3   No. of concentrations/curve 8 8 8 8   Range (ng/mL) 5-100 5-100 5-100 5-100   *Mean slope (SD) 0.0104 (0.0018) 0.0120 (0.0023) 0.0192 (0.0047) 0.0154 (0.0022)   *Mean intercept (SD) -0.0167 (0.0149) -0.0123 (0.0180) -0.0171 (0.0406) -0.0130 (0.0229)   *Mean regression coefficient (SD) 0.9907 (0.0070) 0.9858 (0.0129) 0.9903 (0.0064) 0.9890 (0.0066) Limit of detection (ng/mL)   3.0 3.0 2.0 2.0   Limit of quantification (ng/mL) 5.0 5.0 5.0 5.0 *Each of eight replicates.

Table 1: Regression analysis of calibration curves of assays of vitamin D-2, vitamin D-3, and their 25-hydroxy metabolites.

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