Inter assay variation Intra assay variation
Conc.taken (ng/ml) Conc. found (Mean ± S.D.) (ng/ml) D.F.A
(%)
R.S.D.
(%)
Conc. found
(Mean ± S.D.)
(ng/ml)
D.F.A
(%)
R.S.D. (%)
N
208 222.54 ± 20.56 9.24 6.99 210.77± 27.67 13.13 1.33
616 560.68± 34.65 6.18 -8.98 577.93± 37.64 6.51 -6.18
1040 1024.22± 57.28 5.59 -1.52 1047.98±30.22 2.88 0.77
NCG
206 220.88 ± 23.63 10.70 7.22 219.53 ± 25.61 11.67 6.57
618 669.52 ± 25.05 3.74 8.34 670.31 ± 27.13 4.05 8.46
1030 1095.38 ± 71.20 6.50 6.35 1050.25 ± 64.51 6.14 1.97
R.S.D. = Relative standard deviation
D.F.A. = Deviation from actual concentration
S.D. = Standard deviation
Table 1: Intra and Inter assay validation of N and NCG.
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