Spot No. Protein Name Theoretical MW Theoretical pI Score* Sequence Coverage Ratio Accession No. 2 transportin 1 103091 4.81 102 13 3.80 Q92973 5 splicing factor 3a subunit 3 59238 5.27 76 14 0.46 Q12874 6 phosphogluconate dehydrogenase 53745 6.80 71 12 0.47 P52209 8 thioredoxin domain containing 5 44636 5.77 68 12 0.20 Q8NBS9 9 spermine synthase 24942 5.16 71 19 0.34 P52788 10 annexin A2 38864 7.57 114 28 0.34 P07355 11 otubain 1 31549 4.85 81 26 0.23 Q96FW1 12 DnaJ homolog subfamily C member 8 29823 9.04 89 28 0.42 O75937 13 actin related protein 2/3 complex subunit 2 34454 6.84 71 20 0.49 O15144 14 14-3-3 protein γ 28498 4.80 150 36 0.39 P61981 * Probability-based MOWSE score. Scores higher than 65 indicate the level of statistical significance at p < 0.05.

Table 2: Proteins differentially expressed in cells exposed to carbon black. Proteins were identified using a proteomic approach involving 2-dimensional gel electrophoresis and MALDI-TOF MS. A total of 14 differentially expressed spots were detected, from which 10 proteins were identified. No proteins were identified from spots 1, 3, 4, and 7 [25].

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