analytical methods developed for estimation


Comparison of analytical methods developed for estimation of topiramate in biological matrix.
Sr. No.	Processing volume	Extraction procedures	Evaporation and Reconstitution	Analytical run time (Min)	LOQ (ng/ml)	Detection technique	Reference
1	NA	LLE and derivatization	Done	NA	40	HPLC-UV	1
2	0.1 ml plasma	LLE	Done	NA	2000	LC-MS-MS	2
3	0.5 ml plasma	PPT	Not required	4	250	LC-MS-MS	3
4	0.1 ml plasma	NA	NA	NA	20	LC-MS-MS	4
5	0.1 ml plasma	LLE	Done	NA	2000	LC-MS-MS	5
6	0.1 ml plasma	LLE	Done	6	2000	LC-MS-MS	6
7	NA	NA	NA	NA	16.6	LC-MS-MS	8
8	0.5 ml plasma and blood	PPT	Not required	5	3000	GC-FID	9
9	0.3 ml plasma	SPE	Done	2.5	10.4	LC-MS-MS	10
10	0.5 ml plasma	LLE	Done	>6.0	100	GC-FID	12
11	NA	PPT	Done	2.5	20	LC-MS-MS	13
12	NA	LLE	Done	>5.32	500	GC-NPD	15
13	NA	LLE	Done	>7.2	1000	GC-NPD	16
14	0.5 ml in Serum	LLE	Done	>4.0	2500	GC-NPD	17
15	0.1 ml plasma	SPE	Not required	1.8	10	LC-MS-MS	Present method

LLE: Liquid-Liquid extraction; PPT: Protein precipitation; NA: Not Available

Table 1: Comparison of analytical methods developed for estimation of topiramate in biological matrix

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