steady-state levels and translational

	pEGFP-transfected cells Control E⁺/B48 E‾/B48			S51A-transfected cells Control E⁺/B48 E‾/B48
Total RNA^a
LAL	0.15 ± 0.02	0.21 ± 0.03*	0.18 ± 0.03	0.18 ± 0.02	0.20 ± 0.03	0.22 ± 0.03
Cath B	0.53 ± 0.04	0.69 ± 0.09*	0.57 ± 0.07	0.66 ± 0.09	0.67 ±0.09	0.72 ± 0.08
ATF4	0.20 ± 0.02	0.21 ± 0.03	0.23 ± 0.05	0.18 ± 0.03	0.17 ± 0.03	0.19 ± 0.03
Polysome-associated RNA^b
LAL	0.29 ± 0.02	0.26 ± 0.02	0.21 ± 0.03*	0.29 ± 0.06	0.31 ± 0.04	0.32 ± 0.04†
Cath B	0.24 ± 0.02	0.25 ± 0.04	0.17 ± 0.02*	0.27 ± 0.02	0.26 ± 0.01	0.28 ± 0.01†
ATF4	0.63 ± 0.12	0.64 ± 0.05	0.91 ± 0.11*	0.69 ± 0.11	0.61 ± 0.09	0.73 ± 0.10†

LAL; lysosomal acid lipase, Cath B; cathepsin B, ATF4; activating transcription factor 4
^aSteady-state level of each mRNA was expressed relative to GAPDH mRNA levels.
^bPolysome-associated mRNA levels were normalized with respect to its steady-state
level.
Values represent the mean ± SEM of five experiments.
* P<0.05 as compared with the control, and † P<0.05 as compared with the cells transfected with empty pEGFP vector and treated with EÎ/B48 lipoproteins.

Table 1: Differential regulation of steady-state levels and translational efficiencies of specific mRNAs in Raw 264.7 cells treated with E‾/B48 lipoproteins.

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