| Calibration |
| Linearity range |
0.05 - 5 ng/mL |
| Slope ± S.D. (n=5) |
0.97633 ± 0.03495 |
| C.V.% (slope) |
3.6 |
| r (mean ± S.D) |
0.99984 ± 0.00016 |
| Intercept (mean ± S.D) |
0.01256 ± 0.00458 |
| Limit of quantification (LOQ) |
0.05 ng/mL |
| Limit of detection (LOD) |
0.003 ng/mL |
Intra-assay precision and accuracy (n=5) |
| Conc. (ng/mL) |
Measured mean (ng/mL) ± S.D. |
C.V.(%) |
Mean accuracy (%) |
| 0.05 |
0.04902 ± 0.0024 |
4.9 |
98 |
| 0.10 |
0.09542 ± 0.0029 |
3.1 |
96 |
| 0.25 |
0.22957 ± 0.0052 |
2.2 |
92 |
| 1.0 |
0.88797 ± 0.0205 |
2.1 |
89 |
| 2.5 |
2.23212 ± 0.0379 |
1.5 |
89 |
Inter-assay precision and accuracy (n=5) |
| Conc. (ng/mL) |
Measured mean (ng/mL) ± S.D. |
C.V.(%) |
Mean accuracy (%) |
| 0.10 |
0.10009 ± 0.00948 |
9.4 |
101 |
| 0.25 |
0.24932 ± 0.01437 |
5.8 |
100 |
| 1.0 |
0.93760 ± 0.04746 |
5.1 |
94 |
| 2.5 |
2.32050 ± 0.08518 |
3.7 |
93 |
