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| Figure 2: (A) HPLC-MS chromatograms of 5-Aza-dC and its degradation products. The upper diagram shows the guanylurea derivatives a-pentose (RT: 2.9), bpentose (RT: 3.9), a-hexose (RT: 4.5) and b-hexose (RT: 6.5). The diagram in the middle displays thea-and b-anomers of 5-Aza-dC (RT: 5.8 and 7.4, respectively) and the internal standard dU (RT: 11.3). The lower diagram shows the formylated derivatives (RT: 9.5). (B) HPLC-MS chromatograms of the phosphorylated forms of 5-Aza-dC. From top to bottom are displayed the chromatograms for 5-Aza-dC monophosphate (5-Aza-dCMP), diphosphate (5-Aza-dCDP) (RT: 16.6), triphosphate (5-Aza-dCTP) (RT: 16.5) and the internal standard CTP (RT: 16.5). (C) Calibration curve reporting the correlation of the peak area ratio between 5-Aza-dC/dU and nanograms of 5-Aza-dC. (D) Calibration curve reporting the correlation between the peak area and nanograms of CTP. |
