Cell line

Hypericin concentration

Light Source

Wavelength (nm)

Light dose (J/cm²)

Results

Reference

Human skin carcinoma (A431)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Human cervix carcinoma (ME-180)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Human cervix carcinoma (HeLa)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Human prostate carcinoma (DU 145)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 &0.16 µM

[6]

Human prostate adenocarcinoma (PC-3)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Human breast carcinoma (MCF-7)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Human prostate carcinoma

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 &0.16 µM

[6]

Human fibrosarcoma
(Hs913T)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Mouse fibroblast cells (Swiss 3T3)

100 µM

Fluorescence lamps

530
620

4

IC₅₀
0.10 & 0.16 µM

[6]

Mouse Mammary carcinoma cells,
(EMT6)

0.5 µM
1.0 µM

Fluorescent bulbs

1.5

Nontoxic 0.5 µM + 1.5 J/cm²;
Moderately cytotoxic
1.0 µM + 1.5 J/cm²

[7]

Human lymphoma (U937)

0.2 µM

Argon dye laser

599

2.5
4 -5
8
10
18

No effect (2.5 J/cm²)
Critical effects 
(4-5 J/cm²)
Lethal doses
(8-10 J/cm²)

[8]

Murine C26 colon carcinoma cells

1 - 4 µM s

fibre optic Noncoherent light delivery system

550
590

0.3
0.6
1.2

LD₅₀
1 µM
1.60 J/cm²
55O nm

[9]

Human Squamous oesophageal
(Kyse-140)

10 nM -   1 µM

100W halogen lamp

400  – 800

30

IC₅₀
27nM-28nM

[10]

Human adenocarcinoma
Oesophageal
(OE-33)

10 nM -   1 µM

100W halogen lamp

400  – 800

30

IC₅₀
27nM -28nM

[10]

Human Glio0.blastoma
(U373)

1-100 µM

Argon laser

488

1 - 20

30% killing
100 µM
20 J/cm²

[11]

Human bladder
(T24 & RT)

20 µg/ml
60 µg/ml

Argon-pumped dye laser

630

1 – 2
4
8

LC₅₀
3.6 J/cm²
60 µg/ml

[12]

Osteosarcoma cell line
(U2OS)

0.06-2 µg/ml

300W halogen lamp

2.9
4.8
9.6
14.4

100% killing
1 µg/ml + 9.6 J/cm² ;
0.5 µg/ml + 14.4 J/cm² ;
IC₅₀
0.4 µg/ml +  9.6 J/cm²

[13]

Human breast adenocarcinoma
(MCF-7)

0.021 µM

11 L18 W/30 lamps

530 - 620

4.4

80% survival after 24hrs & 60% survival after 48 hrs

[14]

Human Breast adenocarcinoma
(MCF-7)

0.0084 – 0.021 µM

Lamp

530 – 620

4.4

Greater reduction in cell viability with 48 h

[14]

Human epidermoid carcinoma
(A431)

1 µM

Red light diode array

610

1.5

LD₅₀
0.31 µM

[15]