Name

Extraction system

Amplification system

HIV-1 target region

Amplification and detection method

Assay software

Sample input volume (mL)

Linear dynamic range

Abbott RealTime
HIV-1 assay

Automated m2000sp

m2000rt

Pol IN

Real-time PCR , fluorescent detection

Open mode 0.6mL RNA HIV-1 assay  version 2

0.6

40-1×107 copies/mL

Cobas AmpliPrep-
Cobas TaqMan
HIV-1 assay

Automated COBAS AmpliPrep

COBAS TaqMan

gag

Real-time PCR, fluorescent detection

AMPLILINK 3.1.2

0.85

48-1×107  copies/mL

Reference: (Abbott, May 2007a; Abbott, June 2008; Abbott, May 2007b; Gueudin et al., 2007; Roche, May 2007; Scott et al., 2009)
Table 1: Summary of Assay characteristics.
Goto home»