DrugsClones Amprenavir (2.2)* Atazanavir (2.1)* Darunavir (2)* Indinavir (2.3)* Lopinavir (1.6)* Nelfinavir (2.2)* Saquinavir (1.8)*   IC50 FC IC50 FC IC50 FC IC50 FC IC50 FC IC50 FC IC50 FC CRF02_AGL10I 0.0184 ± 0.0017 0.92 0.0068± 0.0004 0.94 0.0043 ± 0.0002 1.10 0.0175± 0.0092 1.30 0.0230 ± 0.0050 0.78 0.0123± 0.0050 0.83 0.0106 ± 0.0006 0.96 CRF02_AGL10V 0.0177± 0.0004 0.89 0.0069 ± 0.0004 0.96 0.0036± 0.0003 0.92 0.0156 ±0.0074 1.20 0.0220 ±0.0024 0.75 0.0131± 0.0070 0.89 0.0102± 0.0007 0.93 CRF02_AGWT_10L 0.0199± 0.0014 - 0.0072 ± 0.0009 - 0.0039± 0.0005 - 0.0134± 0.0035 - 0.0292 ± 0.0030 - 0.0147± 0.0060 - 0.011±0.0007 - * Fold-change (FC) biological cut-off determined for each PI by Antivirogram-Virco. IC50 values represent the average of two experiments in duplicate. For each drugs, five different concentrations were used. Susceptibility of drugs has been determined by the fold change of IC50 (FC) and calculated as the IC50 ratio CRF02_AGL10I/ CRF02_AGWT_10L and CRF02_AGL10V / CRF02_AGWT_10L.

Table 4: Comparison of IC50 (μM) of mutants CRF02_AGL10I and CRF02_AGL10V vs wild type CRF02_AGwt_10L.

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