|
| Figure 1: Visualization ofgiant cell formation. Persistently HIV-infected HUT-78/IIIB cells were cocultivated with non-infected CD4+ target SupT1 T cells at 37°C. Over 24 h of co culture, every 15 minutes, giant cell formation was recorded by the live cell image JuLITM viewer. Images are shown at 0 h, 5 h, 8 h, 11 h, 14 h, 17 h and 20 h. The SupT1 cells were labeled with the green fluorescent CellVue® Jade dye kit and after 20 h of coculture the green fluorescent dye of the SupT1 cell membranes is present in the HIV-induced multinucleated giant cells (20 h, last picture panel). |
