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| Figure 1: Sensitivity of HIV-1 variants to muparfostat and efficacy of their replication in cells. (A) The viral variants selected for after 24 and 21 passages of HIV-1IIIBstrain in H9 cells in the presence of muparfostat (P24, P21) or in the absence of this compound (C21) as well as original non-passaged (Wild type) virus were tested for sensitivity to muparfostat in H9 cells. (B) The H9 cells were infected with 100 CCID50 of P21, C21, and wild type HIV-1, and the amount of viral p24 released into the culture supernatant medium determined after 6-10 days of incubation of cells. The results are expressed as a percentage relative to mock treated viral control in the presence of muparfostat (Figure 1A) and as μg/mL (Figure 1B) of the amount of viral p24 produced. Results are shown as mean values of three separate experiments. |
