Method Description
ELISA-assisted PNA array Advantages
-No viral RNA purification necessary
-Sensitive (detection limit:104 pfua)
-Detect the presence of a viral gene presence using the naked eye Disadvantages
-ELISA system anti-NP antibody is necessary
-Diagnosis requires 2-3 hours.
Gold-conjugated antibody-assisted PNA chromatography Advantages
-Rapid (result can be obtained in 5 min)
-No virus purification, incubation or washing necessary
-Sensitive (detection limit: 104 pfu)
-Detect viral gene presence by the naked eyes Disadvantages
-Gold-nanoparticle conjugated anti-NP antibody is necessary
aPlaque forming unit
Table 1: Diagnosis of swine-origin influenza A virus (H1N1/2009) by PCR-free viral RNA detection methods.
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