Figure 1 | Design of the study. (a) Protection of gene-modified..

Figure 1: Design of the study. (a) Protection of gene-modified and unmodified HIV target cells by sscFv_PRO140. A lentiviral vector is used to generate producer cells by stably integrating the gene encoding sscFv_PRO140 (orange) into the genome of HIV target (or non-target) cells. Upon translation, sscFv_PRO140 moves from the lumen of the rough endoplasmic reticulum (rER) through the Golgi complex and is released from the cell. Binding of sscFv_PRO140 to surface CCR5 protein should render gene-modified and unmodified HIV target cells non-permissive to R5 HIV.
(b) Design of sscFv_PRO140. sscFv_PRO140 was designed by joining the variable heavy (V_H) and variable light (V_L) chains of mAb PRO 140 with a flexible (GGGGS)3 linker (L₃). VH regions of mAb PRO 140 are shown in red, V_L regions in blue and constant regions in grey. (c) Schematic of the vectors used in this study. The gene encoding sscFv_PRO140 was cloned downstream of the promoter and upstream of the internal ribosome entry site (IRES). LTR, long terminal repeat; psi, packaging signal; RRE, rev response element; S, CD4 signal peptide; H, 6xHis-tag; P_CMV, cytomegalovirus immediate early promoter; cPPT, central polypurine tract; WPRE, woodchuck hepatitis post-transcriptional regulatory element.

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