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Figure 1: Infection/Induction Protocol. BALB/c mice were grouped into six groups. Group 1 received only distilled water from days 1 to 36 and NSS from days 37 to 72 as placebo. Group 2 received A. suum eggs every three days from days 1-36. Group 3 received D. pteronyssinus extract from days 1-10 and booster from days 31-33. Group 4 received A. suum eggs from days 1-36 every three days and D. pteronyssinus extract from days 37-46 and booster dose from days 66-69. Group 5 received D. pteronyssinus extract from day 1-10 and from days 31-33 and received A. suum eggs every three days from days 37 to 72. Group 6 received A. suum eggs every three days from days 1-36 and D. pteronyssinus extract from days 1-10 and from days 31-33. Blood extraction and fecalysis were done at days 1, 36 and 72. At day 72, three mice per group were sacrificed for histopathologic analysis of the lungs, liver and intestines. |