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| Figure 5: Effect of MAG-DHA and MAG-EPA on U46619-induced Ca2+ hypersensitivity and RhoA activation in OVA sensitized guinea pigs. A: CCRC to free [Ca2+] in the presence of 20 nM U-46619 obtained from ß-escin permeabilized tissues in Control (closed circle, n = 15), OVA (open triangles, n = 20), OVA + MAG-DHA (open squares, n = 18) and OVA + MAG-EPA (open circles, n = 16). Each point represents the mean ± SEM, * P < 0.05. B: Rho activation assay was performed on bronchi homogenates derived from Control (untreated), OVA-sensitized, OVA-sensitized + MAG-DHA-treated and OVAsensitized + MAG-EPA-treated guinea pigs. Mean bar histogram displaying the quantitative analysis of various GTP-RhoA density ratios. The ß-actin staining was obtained from the original homogenates derived from the corresponding preparations, n = 5, * p < 0.05. |
