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| Figure 1: Septic LPA2-/- mice showed similar manner as wild type in plasma IL-6 level, BAL IL-6 level, cell numbers, and protein levels in lungs, except reduction of plasma KC levels – A, C. Wild type and LPA2-/- mice were challenged with CLP for 24 h. Plasma IL-6 and KC levels were measured by a ELISA kits. Data represent mean ± SD and n = 4-7. *p < 0.01 vs sham mice, **p < 0.05 vs sham wild type. B, D. Wild type and LPA2-/- mice were challenged with CLP for 24 h and BAL fluids were collected. BAL IL-6 and KC levels were measured by mouse cytokine ELISA kits. Data represent mean ± SD and n = 4-7. *p < 0.01 vs sham mice. E. BAL cell number was accounted. Data represent mean ± SD and n = 4-7. F. BAL protein concentration was measured with BSA as series standard. Data represent mean ± SD and n = 4-7. G. mouse LPA2 mRNA expression was examined by Real-time PCR. Data represent mean ± SD and n = 3-4. |
