Antibody

Host

Dilution

Reaction conditions

First

Biotinylated anti-mouse Ly-6G Neutrophil marker monoclonal

Rat

1:50

4 °C, overnight

Anti-mouse F4/80 monoclonal

Rat

1:50

4 °C, overnight

Anti-mouse versican (GAG beta domain) polyclonal

Rabbit

1:1000

4 °C, overnight

Second

Alexa Fluor 488-conjugated streptavidin

Goat

1:2000

RT, 1 h

DyLight 546-conjugated anti-rat IgG

Goat

1:5000

RT, 1 h

Alexa Fluor 546-conjugated anti-rabbit IgG

Goat

1:2000

RT, 1 h