sample

dcw (mg/ml)

Volume of cell sample used for extraction (ml)

Concentration of RNA product (ng/µl)

Amount of RNA used for cDNA synthesis (µl)

Calculated amount of synthesized cDNA in 20 µl reaction (ng/µl)

T10

0.9

23

336.1

1.5

504

T24

1.80

11.5

704.4

0.7

493

T36

2.89

7.2

152.4

3.3

503

T60

4.60

4.5

37.7

13.3

501

Table 2: Dry cell weight (dcw) and normalization of amounts of cells for RNA extraction and cDNA synthesis.
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