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Figure 2: Image acquired with the RPI method. The surface of a prism of perfluorinated material isorefractive with water was coated with a functional copolymer enabling the immobilization of spot (200 m diameter) of different antibodies toward p24 capsid protein (upper and lower rows) and hepatitis B surface antigen (middle row). The amount of target molecules binding on the spots is directly obtained from the local brightness of the image, representing the intensity of light reflected by the surface. Adapted from Ref. [1]. |