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| Figure 4: Phenotypic characterization of HAPCs- derived iPSCs. a) Morphology of HAPCs-iPSCs. Colonies depicted the compact colony structure resembled mouse ESCs on day 14 days after the treatment of electroporation. b) Alkaline phosphatase staining of HAPCs-iPSCs. c, d) Imunocytochemical analysis of pluripotency marker, Oct4 and Sox2. e) RT-PCR analysis of transcripts from pluripotency genes for Oct4, Sox2, Klf2, c-Myc, hTERT and Nanog in HAPCs-iPSCs. Magnification in a-d) ×200. |
