(A) PC12 cells were treated with 5µM K-350 for the indicated times in serumfree DMEM. nur77 and beta actin (control) mRNAs were detected by RT-PCR.
(B) Semiquantitative analysis of nur77 gene expression. For nur77 cDNAs, the total area of white pixels and their intensity were determined by using CS Analyzer 1.0 software. PCR amplification was performed for 22 (black), 24 (white), 26 (gray), 28 (stripe), 30 (dot) and 35 (horizontal stripe) cycles.
(C) Semiquantitative analysis of nur77 gene expression after treatment with K-350 for 2 h.
