a) Collagen IV concentration (pg col IV/ug total protein) for wild type (wt) fibroblasts in NF and NF+MMP scaffolds at days 1, 6 and 14. Collagen IV levels at day 1 were higher in NF than in NF+MMP scaffolds and decreased with time, indicating ECM remodeling.
b) Collagen IV concentration (pg col IV/ug total protein) for diabetic (db) fibroblasts in NF and NF+MMP scaffolds at days 1, 6 and 14. Collagen IV levels were highest at day 1 in NF scaffolds, with no effect of exogenous MMP-2 (NF+MMP). Expression levels were normalized using total protein content as measured using Bradford assay. + p<0.05 when compared to NF samples, ^p<0.05 when compared to day 1 samples of same experimental group.
