RH group 1: Marker names Relative position in the RH group Distance between markers Cumulative distance Theta (%) 2pt LOD Ma 23 1 0.0 cR       Ma 25 2 69.4 69.4 cR 50.0 % 3.2 Ma 26 3 15.0 84.4 cR 13.9% 10.8 Ma 27 4 9.9  94.3 cR 9.4 % 11.8 Ma 32 5 28,9 123.2 cR  25.1 % 6.9 Ma 33 6 35,2 158.4 cR 29.6 % 6.3 Ma 34 7 21,2 179.6 cR 19.1 % 9.0 RH group 2 : Marker names Relative position in the RH group Distance between markers Cumulative distance Theta (%) 2pt LOD Ma 8 1 0.0 cR       Ma 6 2 68.2 cR 68.2 cR 49.4 % 1.3 Ma 3 3 16.8 cR 85.1 cR 15.5 % 5.0 Ma 1 4 16.9 cR 102.0 cR 15.5 % 7.1 Ma 2 5 48.4 cR 150.4 cR 38.4 % 5.5 Ma 5 6 55.5 cR 205.8 cR 42.6 % 4.3 3rd group : Marker names Relative position in the RH group Distance between markers Cumulative distance Theta (%) 2pt LOD Ma15 1 0.0 cR       Ma19 2 24.8 cR 24.8 cR 22.0 % 6.4 Ma 20 3 53.7 cR 78.5 cR 41.6 % 4.7 Ma21 4 61.4 cR 139.9 cR 45.9 % 3.0 Ma17 5 37.1 cR 177.0 cR 31.0 % 3.8 Ma18 6 53.3 cR 230.3 cR 41.3 % 3.8

Table 7: Order of markers within the three largest RH groups. Multipoint analyses were carried out with CarthaGene software. Distances are expressed in centirays (cR3500) (Schiex et al. 1997). The order of markers as defined by the sequence nucleotide (1st column) appear to be exactly the same as that defined by RH mapping (2nd< column) for RH group 1. In RH group 2 and 3, there are a small number of inversions between the two methods but overall the two mapping strategies gave collinear results all along the 20 Kb of BAC188K9 analyzed.

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