fertilization rate of eggs of

Name of stimulant	Dose		Mean No. of fertilized eggs and rate of fertilization (%)
Name of stimulant	Male	Female	26°C		28°C		30°C
Pituitary gland extract	25 mg/kg 50 mg/kg	40 mg/kg 120 mg/kg	1994^bm ± 29 4134^dn ± 51	46% 76%	2215^ao ± 30 4524^do ± 33	50% 80%	2126^an ± 35 4026^cm ± 58	48% 72%
0.4 ml/kg	0.4 ml/kg 1.0 ml/kg	0.8 ml/kg 2.0 ml/kg	1860^am ± 53 3827^cm ± 40	41% 72%	2325^bo ± 37 4212^cn ± 37	45% 77%	2093^an ± 36 3780^bm ± 31	45% 70%

Values (mean ± SD) within columns indicated by different superscript letters (a,b,c,d) and values (mean ± SD) within rows indicated by different superscript letters (m, n,o) are significantly different (DMRT, p<0.05).

Table 3: Effects of stimulants and temperature on fertilization rate of eggs of Clarias batrachus.

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