| Gene |
Primers |
| StAR |
Forward 5’-TGGGGAAGGTGTTTAAGCTG-3’ |
| |
Reverse 5’-AGGGTTCCAGTCTCCCATCT-3’ |
| P450ssc |
Forward 5’-GCTTCATCCAGTTGCAGTCA-3’ |
| |
Reverse 5’-CAGGTCTGGGGAACACATCT-3’ |
| β-Actin |
Forward 5’-TGTCCCTGTATGCCTCTGGT-3’ |
| |
Reverse 5’-AAGTCCAGACGGAGGATGG-3’ |
| EF-1α |
Forward 5’-CATTGACAAGAGAACCATTGA-3’ |
| |
Reverse 5’-CCTTCAGCTTGTCCAGCAC-3’ |
Abbreviations: EF-1α, elongation factor 1α; P450ssc, P450 side chain cleavage;
StAR, steroidogenic acute regulatory.