CS30 CS36 CS37 F. pectinovorum F. hydatis Gliding Motility + + + +1 +2 Flexirubin Type Pigment + + + +1 +1 Congo Red Absorption - - - -2 -2 Growth at 25ºC + + + +2 +2 Glucose Utilization - - - +2 +2 TSI Reaction K/N/-/- K/N/-/- K/N/-/- • • Lysine Decarboxylase - - - • -1 Ornithine Decarboxylase - - - • -1 Arginine Dihydrolase - - - • • Phenylalanine Deaminase + - - • -1 Esculin Hydrolysis + + + +2 +2 Cytochrome Oxidase - - - +1 -1 Catalase + + + +1 +1 Lipase + - - +1 +1 Gelatinase + + + +1 +1 Caseinase + + + +2 +1 Starch Hydrolysis + + + +2 +1 Urease - - - -1 -1 Agar Degradation - - - -1 -2 ONPG + + + +2 +2 Susceptibility to 0129 - - - +1 -1 Simmons Citrate - (+) (+) • • Production of Indole - - - -1 -1 Production of H2S - - - -1 -1

Table 1: Biochemical characteristics of representative Flavobacterium spp. strains CS30, CS36 and CS37 recovered from coho salmon. Tests were inoculated with 24 hr old subcultures and incubated at 22ºC for up to 7 days. Results are presented as + for positive reaction; - for negative reaction; and (+) for weak positive reactions. For the TSI reaction, results are expressed as K/N/-/- designating alkaline slant/nonreactive butt/no production of H2S/ no production of gas. For comparative purposes, biochemical characteristics of both Flavobacterium pectinivorum and F. hydatis are included as reported by 1: Reichenbach (1989) and 2: Bernardet and Bowman (1996) or *: not reported

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