Phylogenetic group Band no. Accession no. Relative abundance (RA, %)2 Closest relative (obtained from BLAST search) Identity (%) Auto-CI1 Allo-CI Auto-MAP Allo-MAP Auto-CG Allo-CG Actinobacteria    34 FJ235661 - - - 5.5 - - Arthrobacter arilaitensis (EU834260) 100 49 FJ235688 - - - - - 12.8 Arthrobacter arilaitensis (EU834260) 98 35 FJ235683 9.5 2.3 - - - - Corynebacterium sp. (EU798947) 100 4 FJ235642 0.8 - 6.3 3.2 22.1 9.8 Luteococcus sanguinus (AJ416758) 98 Firmicutes         32 FJ235659 - - - - 4.5 - Anoxybacillus flavithermus (EU816689) 99 36 FJ235662 - - 1.2 - 21.3 - Anoxybacillus flavithermus (EU816689) 100 30 FJ235658 1.3 - 16.9 - - - Azospirillum oryzae (DQ682470) 100 26 FJ235681 1.8 - - - - - Bacillus arsenicus (EU624418) 100 27 FJ235656 2.3 9.6 2.5 - - - Bacillus cereus (EU439436) 93 25 FJ235680 - - 5.2 3.2 9.3 0.5 Bacillus sp. (DQ861214) 99 48 FJ235687 - 0.7 - - - - Clostridium sp. (AY949856) 100 1 FJ235671 - - - - 3.0 22.4 Lactococcus lactis subsp. (AM944595) 100 3 FJ235641 - 5.2 13.4 3.1 - - Staphylococcus sp. (EU734673) 99 Proteobacteria       15 FJ235648 - 6.9 - - - - Acinetobacter sp. (EU263292) 100 39 FJ235663 0.5 22.5 0.7 3.4 14.5 18.4 Agrobacterium sp. (FJ796437) 98 24 FJ235655 - 1.6 - 23.7 1.6 - Enterobacter cloacae (EU554428) 99 17 FJ235649 1.5 - - 22.0 1.3 - Klebsiella sp. (EU294414) 100 8 FJ235673 3.7 - - 9.6 - 3.7 Kluyvera cryocrescens (AM992189) 98 31 FJ235682 - - 15.3 1.4 - - Pectobacterium wasabiae (EU490611) 100 11 FJ235676 1.4 1.5 - 13.5 - - Uncultured α- Proteobacterium (EF629637) 94 Unclassified bacteria              2 FJ235640 14.9 - - - - - Uncultured bacterium (EF669501) 100 5 FJ235643 4.1 9.5 18.7 2.0 22.4 11.2 Uncultured bacterium (EU789853) 100 6 FJ235644 12.8 0.8 - - - - Uncultured bacterium (EU789853) 99 7 FJ235672 15.5 1.4 0.7 - - 10.8 Uncultured bacterium (EF669501) 98 12 FJ235645 2.1 0.9 13.8 2.6 - - Uncultured bacterium (EU527100) 99 14 FJ235647 14.5 1.2 - - - - Uncultured bacterium (EU289492) 100 16 FJ235678 12.7 6.3 - - - - Uncultured bacterium (EF669501) 99 18 FJ235678 0.5 - - - - - Uncultured bacterium (EU804054) 100 19 FJ235651 - - 5.2 - - - Uncultured bacterium (EU790458) 100 20 FJ235652 - 13.6 - - - 3.8 Uncultured bacterium (EU773842) 100 33 FJ235660 - - - 6.9 - - Uncultured eukaryote (EF188563) 98 40 FJ235685 - 16.0 - - - - Uncultured bacterium (EU774619) 98 50 FJ235689 - - - - - 6.7 Uncultured bacterium (EU826726) 99                     Uncultured Actinobacteria  56 FJ429642 - - - 6.1 26.4 19.1 Uncultured actinobacterium (EU852548) 95 54 FJ429641 13.2 19.1 6.1 - 35.0 44.7 Uncultured bacterium (AY963375) 92 Proteobacteria  52 FJ429649 - - - - - 22.0 Hafnia sp. (EU159563) 99 55 FJ429647 47.5 80.9 2.2 18.5 38.6 14.2 Ochrobactrum tritici (EU999218) 99 Undetermined actinomycetes      U1   - - - 15.3 - -     U3   - - 8.9 - - -     U4   - - 6.2 - - -     U5   39.3 - - 25.1 - -     U7   - - 76.6 35.0 - -                         Ascomycota            82  FJ236962 - 10.1 - 5.7 14.5 10.1 Aspergillus penicillioides (AB002078) 99 80  FJ236961 9.3 - - - 8.0 12.4 Aspergillus proliferans (AB002083) 100 63  FJ236943 0.2 2.3 - - - - Candida pseudoglaebosa (AB013544) 95 69  FJ236950 0.7 2.3 - - - - Candida pseudoglaebosa (AB013544) 98 79  FJ236960 7.5 12.8 - 0.6 7.3 13.7 Candida pseudoglaebosa (AB013544) 99 89  FJ236968 7.3 12.4 - 1.7 15.1 10.2 Candida silvae (AB053248) 99 90  FJ236969 12.1 20.6 - 17.5 20.2 28.7 Candida solani (AB054566) 99 87  FJ236977 5.1 - - - 4.6 - Komagataella phaffii (EF550394) 98 83  FJ236964 11.0 3.3 42.6 1.0 2.6 0.5 Pleospora herbarum (U43458) 99 84  FJ236976 7.3 8.1 57.4 43.0 14.7 10.2 Uncultured soil ascomycete (AJ515170) 95 70  FJ236951 2.5 0.9 - - - - Yarrowia lipolytica (AF156969) 99 77  FJ236958 7.3 - - - - - Yarrowia lipolytica (AF156969) 100 Basidiomycota  61  FJ236941 0.3 2.2 - - - - Cryptococcus curvatus(AB032626) 99 78  FJ236959 5.2 - - 11.5 - - Trichosporon laibachii (AB001760) 100 Zygomycota  62  FJ236942 0.4 2.3 - - - 1.2 Mucor circinelloides (EU793999) 93 93  FJ236972 - - - - 0.1 - Cochlonema euryblastum (DQ520640) 88 Apicomplexa 66  FJ236948 0.5 - - - - - Eimeriidae environmental sample (EF023121) 92 Choanozoa 71  FJ236952 4.0 6.7 - - 5.4 2.1 Ichthyophonida sp. (AJ130859) 98 Rotifera 94  FJ236973 - - - - - 4.0 Brachionus plicatilis (U49911) 99 Unclassified fungus    65  FJ236945 0.8 - - - - - Uncultured eukaryote (EU326665) 99 67  FJ236946 0.7 2.2 - - 2.1 0.5 Uncultured fungus (AM114819) 99 73  FJ236953 8.4 3.1 - 12.9 5.4 6.4 Uncultured eukaryote (EU326683) 98 81  FJ236963 9.4 10.7 - 6.1 - - Uncultured fungus (EU733595) 97 1Allo, allochthonous; auto, autochthonous; CI, grass carp (C. idella); MAP, bluntnose black bream (M. amblycephala); CG, gibel carp (C. gibelio). 2Relative abundance (RA, %) was represented by the percentage of a specific band intensity to the total band intensity.

Table 1: Representatives of gut microbiota or clones and their relative abundance in polycultured grass carp, bluntnose black bream and gibel carp based on 16S/18S rDNA PCR- DGGE1.

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