Parameter |
- |
0 |
+ |
Methanol content in mobile phase, constant Acetonitrile (mL) |
285 |
300 |
315 |
Acetonitrile content in mobile phase, constant Methanol (mL) |
285 |
300 |
315 |
Mobile Phase A (mL) |
210 |
225 |
240 |
Wavelength (nm) |
229 |
224 |
221 |
Column Oven Temperature (°C) |
20 |
25 |
30 |
TFA Content (mL) |
0 |
0.5 |
1.0 |
Alkyl Sulfonic Acid Sodium Salt (M) |
0 |
0.01 |
NP |
|
