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| Figure 2: HPLC chromatogram obtained during simultaneous determination of SS and TP. Chromatographic conditions: Phenomenex C18 column (250 mm × 8 mm i.d., particle size 10 µm); flow rate 1.2 mL min-1; mobile phase acetonitrile and phosphate buffer 65:35 (v/v) (pH 4.2 ± 0.02, adjusted with triethylamine) and UV detection at 235 nm. |
