Parameter Value
Source temperature (°C) 350
Dwell time per transition (milli seconds) 200
Gas flow/Drying Gas (L/min) 6
Nebulizer gas (psi) 25
Fragmentor (V) 200
Collision energy (V) −10
Ion spray voltage (V) −5500
Mode of analysis Negative
Ion transition for Tacrolimus m/z 802.4→560.2
Ion transition for Pimecrolimus m/z 808.4→548.2
Table 1: Tandem mass spectrometer main working parameters.
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