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Figure 3: Chromatogram of formoterol fumarate and mometasone furoate in metered dose inhalation formulation. Mobile phase: acetonitrile/sodium dihydrogen phosphate buffer-pH 3.0 (50:50, v/v), flow rate–1.0 mL/min, injection volume-100 μL, Column temperature 40°C. Column: C18, 5 μm and 15.0 cm-length, 4.6 mm-inner diameter, UV detection: 220 nm. Peaks asymmetry and theoretical plates of formoterol fumarate peak are 1.30 and 2758, and mometasone furoate peak are 1.18 and 8657, respectively.
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