| Parameters |
N |
T |
Res |
N |
T |
Res |
N |
T |
Res |
| ROS |
ALP |
DCL |
| pH |
3.3 |
3395 |
1.54 |
0.14 |
3886 |
1.51 |
1.96 |
4625 |
1.45 |
1.10 |
| 3.4 |
3369 |
1.51 |
0.24 |
3857 |
1.47 |
1.96 |
4574 |
1.43 |
1.12 |
| 3.5 |
3234 |
- |
0.12 |
3579 |
1.61 |
1.90 |
4403 |
1.37 |
1.09 |
| Mobile phase
(MeOH:H2O) |
78:22 |
2833 |
- |
0.39 |
3035 |
- |
1.08 |
3678 |
1.21 |
- |
| 80:20 |
2630 |
- |
0.36 |
2845 |
- |
1.04 |
3321 |
1.23 |
1.34 |
| 82:18 |
2536 |
- |
0.48 |
2681 |
0.97 |
1.00 |
3026 |
1.18 |
1.90 |
| Flow rate
(mL min-1) |
0.9 |
3049 |
1.44 |
0.73 |
3387 |
1.37 |
1.14 |
4029 |
1.21 |
1.09 |
| 1.0 |
2761 |
- |
0.13 |
3143 |
- |
1.03 |
3795 |
1.22 |
1.87 |
| 1.1 |
2614 |
1.33 |
0.69 |
2779 |
- |
1.02 |
3160 |
1.11 |
1.58 |
N=Theoretical plates, T=Tailing factor, Res=Resolution |
