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| Figure 3: HPLC chromatograms of (A) Typical blank (solvent) baseline in which the solvent peak appeared within the first two minutes followed by the unstable meanders 8 min as the result of gradient elution and disappeared upon reconditioning of the column; (B to D) were alpha-mangostin reference standards of 0.2 μg/mL, 20 μg/mL and 100 μg/mL, respectively; (E) Own isolated, pure alpha-mangostin 100 μg/ml and (F) Extracted alpha-mangostin from the PLGA microspheres. The results of retention time (RT), number of theoretical plate (N) and peak height (H) were expressed as the mean of three replicates ± standard deviation. |
