Parameter Method condition Rt % RSD of peak area
PRG MC PRG MC
Flow rate 0.9 ml/min 6.98 8.56 0.69 0.87
1.1 ml/min 5.21 6.80 0.97 0.49
Mobile phase ratio Water:methanol 55 : 45 5.10 6.75 0.54 0.54
65 : 35 7.10 8.70 0.71 0.59
Table 3: Results from the robustness study of method.
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