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| Figure 1: SDS gel electrophoresis on eluted material after immunoprecipitation of an LSL from AFA is shown in the center lane labeled “AFA.” This ligand was affinity-purified from AVA-W by paramagnetic Dynabeads covalently linked with the fusion protein rHuL-selectin/lgG Fe chimera and eluted from the beads by alkaline treatment at pH 12. The eluted material was subjected to SDS gel electrophoresis under reducing conditions. The negative control, as shown in the lane labeled “C,” was prepared by incubating the Dynabeads coated with the fusion protein rHuL-selectin/lgG Fe chimera with PBS instead of AFAextract. The molecular weight standards included bovine serum albumin with a molecular weight at 66 kDa, as indicated by the arrow in the lane labeled “MW.” Two bands are seen with apparent molecular weight of 57 and 54 kDa, reactivity. The data shown are representative of 12 independent experiments. |
