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| Figure 1: Optimization of albumin-facilitated lipofection formulation. (A) Agarose gel electrophoresis assessment the complex formation in the formulations containing varying amounts of DC at a fixed amount of pCMVβ (1 μg). (B) Panc 1 cells were transfected for 5 h with formulations containing 1 μg DNA plus the various amounts of DC indicated and assayed for β-galactosidase activity in the cells after cultured for 48 h. (C) Agarose gel electrophoresis assessment of the complex formation in the formulations containing various amounts of HSA with 5.5 μg DC and 1 μg DNA. (D) Panc 1 cells were transfected for 5 h with formulations containing 5.5 μg DC, 1 μg DNA plus different amounts of HSA from 0.05 to 40 μg. The data are expressed as nanogram β-galactosidase per microgram of total cell protein (mean ± standard error (SE) obtained from triplicate wells). |
