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| Figure 4: Localization of transfection complexes in transfected Panc 1 cells by confocal microscopy. Panc 1 cells were transfected with the standard formulation prepared with FITC-HSA plus DC plus CX-Rhodamine (Rh)-DNA, then incubated for an additional 6 h with complete culture medium. (A) FITC and Rh overlays of the 3- to 11-μm sections of 1 μm serial Z-sections starting from the bottom of the cells. (B) Composite image of the differential interference contrast, FITC, and Rh at the 8-μm Z-section. The arrowheads indicate colocalization of HSA(FITC) and DNA(Rh) inside the nucleus and the arrows indicate the complexes in the cytoplasm and/or perinuclear space. |
