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| Figure 2: 58 cells expressing murine γc with two myc-tags can be efficiently depleted by anti-myc ab and complement in vitro. (A) CDC assay of wt and myc-tagged murine γc-transduced 58 cells was performed by subsequent incubation with anti-myc ab and complement factors (solid line, black numbers), or complement alone as a control (tinted, grey numbers). Dead cells were detected with PI and quantified by flow cytometry. (B) Calculated specific lysis of the differently tagged cells. |
