|
| Figure 5: Myc-tagged human γc delivers proliferation signals comparable to wt γc in a SCID-X1 B cell line. A γc-deficient human SCID-X1 B cell line was transduced with Li-doubleMYC human γc and enriched for myc expression by MACS. Wt γc-transduced cells were used as a control. For analysis of proliferation, 1 x 106 SCID-X1 B cells were seeded on day 0 and were grown in culture medium containing 15% FCS. After an adaption phase, cells were counted daily. |
