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| Apoptosis labeling revealed low numbers of apoptotic cells (blue nuclei; 6.7 ± 1.7%), including cardiomyocytes, in freshly cut slices of adult rat hearts (A). The number of apoptotic cells increased proportionally with the number of days of culture (E), 16.0 ± 3.2% after 1 day (B), 31.8 ± 1.0% after 3 days (C) and 59.1 ± 8.3% after 6 days (D). Sections counterstained with Nuclear Fast red. Bar markers = 50 μm. n = 4 (one slice per heart) at each time point. |
| Figure 2: Apoptosis of cultured adult heart slices |
