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| Figure 5: Humanins regulated UPR expression in LN18 cells. The cells were incubated with 4 μM humanin M, HNG, HN10d or HN10dV for 24 hours, out of which the last 18 hours they were exposed to Aβ in the sample wells. At the end, the cells were fixed with 4% paraformaldehde, and relative protein expression was determined using appropriate primary and secondary antibodies. All the results were normalized to the control cells without HNs. The protein expression is presented as a mean of relative fluorescence of a particular sample normalized to the control cells +/- variance. The statistical significance was set at p < 0.05 (*), p < 0.01 (**) or p < 0.001 (***). A) Grp94 expression, B) Calregulin expression. |
