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| Figure 3: (A) Immunoflourescence staining for insulin (FITC) and C-peptide (FITC) in Human BM-MSCs and UC-MSCs after pancreatic differentiation. Only in BM-MSCs with or without rat PCs at the end stage of differentiation, islet-like cell clusters were detected which was positive for Insulin and C-peptide (X100). (B,C) UC-MSCs didn’t show any cluster formation and spindle like cells were still observed at the end of differentiation. (D) Human cadaver pancreas was tested as positive control (A) and undifferentiated MSCs were tested as negative control. (E) Nuclei were counterstained with DAPI. |
