| Annealing temperature (°C) |
Sequence (5'-3') |
Strand |
Gene |
|
60 |
CTCATTTCCTGGTATGACAACGA |
F |
GAPDH |
| CTTCCTCTTGTGTTGCT |
R |
| 60 |
AAGAGGCCATCAAGCAGATCA |
F |
Insulin |
| CAGGAGGCGCATCCACA |
R |
| 60 |
GTTTGAGGATTCCTGAGTGTG |
F |
Glut2 |
| GGAAATGTCAAAGGAGACGAT |
R |
| 60 |
CGATATTGTCAGCCGTCTTCTAA |
F |
Nkx2.2 |
| TGCCACCAGTTGTCAGAA |
R |
| 60 |
GACAAAGACGGGAAGAGAA |
F |
Nkx6.1 |
| GGAACCAGACCTTGACCT |
R |
|
