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Figure 2: The quantitative analysis of lipid accumulation in lipid droplets during SVF differentiation using Oil Red O (A) and BODIPY 492/503 staining (B). SVF cells were incubated for 48 hours in adipogenic MDI medium for initiation of differentiation (Ctrl (+) 48 h) and then without differentiation factors for 15 days (Ctrl (+) 15d). The negative control cells (Ctrl (-)) were incubated without differentiation factors. Significance: p<0.01; Ctrl (+) vs Ctrl (-) 15d n=5. |