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Figure 2: Replication Profile over one cell cycle Cells are treated with demecolicine and released. After about 4 hours synchronous cell divisions are observed in all cases at time zero. In the experiment shown, 80% of K562, 60% of THP1, and 50% of MonoMac6 revealed microscopically visible mitotic chromosomes. Numbers are normalized to mol 3HT incorporation into DNA of one cell. Four independent samples are taken for each do. The experiment is performed once. |